Enid Markey was born in Dillon, Colorado. Her first film role was in The Fortunes of War (1911). During the production of The Wrath of the Gods (1914), Markey, a "leading lady with the New York Motion Picture Company", was "badly injured" during the production. During her scene in which the lava flow destroys the village she was surrounded by smoke and fumes and nearly asphyxiated, but had recovered by May 1914.
Her last appearance was in The Boston Strangler (1968).
During the 1950s and 1960s she appeared in several television guest-starring roles, including The Andy Griffith Show as Barney Fife's landlady, and an episode of Gomer Pyle, U.S.M.C., as Grandma Pyle.
In the 1960-1961 season, Markey was cast as Aunt Violet Flower in CBS's Bringing Up Buddy, co-starring Frank Aletter and Doro Merande.
Markey and Merando played spinster aunts who provide a home for their bachelor nephew stockbroker, Buddy Flower, played by Aletter.
